Sedimentation of generalized systems of interacting particles. II. Active enzyme centrifugation--theory and extensions of its validity range.

Abstract

AbstractThe first detailed application of a recently published very general approach to chemical equilibria during sedimentation is presented. As a consequence of the very extensive theoretical treatment, made possible by this approach, the active enzyme analytical centrifugation method can now be used under a far wider set of conditions than before, including the study of many interacting active molecule systems. It has also been shown that this method is as precise as the more conventional ones.