Sediment characteristics mediate mixture effect of metconazole and thiacloprid on the activity behavior of the amphipod Hyalella azteca

Abstract

Pesticide mixtures occur frequently in freshwaters. Here, pesticides can persist over long periods and alter aquatic communities and ecosystems by causing chronic indirect effects. Particularly effects on activity behavior of organisms can be considered as starting points of cascading effects as they provide the basis for further sublethal responses such as reproduction or feeding. Therefore, the impact of two pesticides in combination, the fungicide metconazole and the insecticide thiacloprid, was evaluated on the immobilization and activity behavior of Hyalella azteca with varying sediment conditions. The results showed a change from additive effects to synergism in the mobility tests for sediment with higher contents of total carbon but not for the activity behavior tests using a Multispecies Freshwater Biomonitoring system. However, sediments with high carbon, nitrogen and phosphorous contents led to comparable activity behavior of H. azteca to control conditions after three days of contaminant exposure which was not the case in all other treatments. The autoregressive integrated moving average (ARIMA) forecast approach used showed that this activity behavior remained constant after recovery to pre-exposure levels at least for a time period of 16 h. This study showed that mobility and activity of H. azteca are largely affected by the exposure to pesticides, which is mediated by the structure of the sediment. However, further studies are needed that test activity behavior impairments in environments where the individuals are in direct contact with the sediment that may buffer the pesticide exposure from the water column.