Abstract
This chapter explains the preparation of DNA from eukaryotic cells. This method provides a high-quality preparation of high molecular weight genomic DNA that is suitable for Southern blotting and library construction. Proteinase K is used to liberate nucleic acids from cells, followed by removal of RNA by RNase A. This method is used primarily with cultured cells. The chapter lists the reagents which are used in this process, such as phosphate-buffered saline (PBS), SS-phenol, chloroform, ethanol, TE buffer, and many more. Cells are grown for DNA extraction in advance. Tissue culture cells of various types work well. The chapter also explains DNA extraction.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
