Abstract
Radial glial cells (RGCs) are the most abundant macroglia in the teleost brain and have established roles in neurogenesis and neurosteroidogenesis; however, their transcriptome remains uncharacterized, which limits functional understanding of this important cell type. Using cultured goldfish RGCs, RNA sequencing and de novo transcriptome assembly were performed, generating the first reference transcriptome for fish RGCs with 17,620 unique genes identified. These data revealed that RGCs express a diverse repertoire of receptors and signaling molecules, suggesting that RGCs may respond to and synthesize an array of hormones, peptides, cytokines, and growth factors. Building upon neuroanatomical data and studies investigating direct neuronal regulation of RGC physiology, differential gene expression analysis was conducted to identify transcriptional networks that are responsive to the conserved secretogranin II-derived neuropeptide secretoneurin A (SNa). Pathway analysis of the transcriptome indicated that cellular processes related to the central nervous system (e.g., neurogenesis, synaptic plasticity, glial cell development) and immune functions (e.g., immune system activation, leukocyte function, macrophage response) were preferentially modulated by SNa. These data reveal an array of new functions that are proposed to be critical to neuronal-glial interactions through the mediator SNa.
Highlights
Radial glial cells (RGCs) are a progenitor subtype in the developing central nervous system (CNS)[1] and have a bipolar morphology with radial fibers that serve as scaffolds for neuronal migration[2]
Previous reports in fish have identified dopaminergic[16,17,18] and serotonergic regulation[19] of RGC physiology. Expanding on these neuronal-RGC interactions, our recent study highlighted the neuroanatomical relationship between RGCs and soma of magnocellular and parvocellular neurons immunoreactive for the secretogranin II (SgII)-derived neuropeptide secretoneurin A (SNa) in the goldfish preoptic nucleus[20]
RGCs express an array of hormone, neurotransmitter, and neuropeptide receptors, suggesting a multiplicity of new functions critical to neuronal-glial communication
Summary
Radial glial cells (RGCs) are a progenitor subtype in the developing central nervous system (CNS)[1] and have a bipolar morphology with radial fibers that serve as scaffolds for neuronal migration[2]. RGCs are mostly a transient cell type, differentiating into neurons and glia at the end of development. They only persist in two areas of the adult brain, the anterior part of the subventricular zone of the lateral ventricle and subgranular zone of the dentate gyrus, where they serve as progenitor cells. Previous reports in fish have identified dopaminergic[16,17,18] and serotonergic regulation[19] of RGC physiology Expanding on these neuronal-RGC interactions, our recent study highlighted the neuroanatomical relationship between RGCs and soma of magnocellular and parvocellular neurons immunoreactive for the secretogranin II (SgII)-derived neuropeptide secretoneurin A (SNa) in the goldfish preoptic nucleus[20].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
