Abstract
Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF-β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.
Highlights
For almost the last two decades, researchers have focused on the study of mesenchymal stem cells (MSCs) due to their specific biological features
The conditioned media from human and rabbit Adipose tissue-derived MSCs (AT-MSCs), human umbilical vein endothelial cells (HUVECs), and rabbit endothelial progenitor cells (EPCs) were collected after 24 h of culture
As shown in the heat map (Figure 1B), only a few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2)
Summary
For almost the last two decades, researchers have focused on the study of mesenchymal stem cells (MSCs) due to their specific biological features This specific cell type can be presently obtained from various human as well as rabbit biological sources, most often from bone marrow, amniotic fluid, or others [1,2,3,4]. Adipose tissue-derived MSCs (AT-MSCs) are very interesting for the cellular therapy, as they show an immuno-regulatory ability via cytokine secretion or the expression of cytokine receptors [6] Their immunomodulatory effect is similar to that of MSCs derived from bone marrow [7], indicating a greater stem cell-based immune-therapeutical potential of AT-MSCs. In addition, MSCs in general might control neovascularization and secret different angiogenic, anti-apoptotic, immunosuppressive, or proliferation-stimulating factors [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
