Abstract
Background:Asparaginyl endopeptidase, also known as legumain (EC 3.4.22.34) shows strong activity in the mouse kidney. Legumain is also highly expressed in tumors. DJ-1/PARK7 is a Parkinson’s disease- and cancer-associated protein. DJ-1 is a coactivator of various transcription factors. Recently, we reported that transcription of the legumain gene is regulated by p53 through DJ-1.Methods:We measured the secretion levels of legumain in a conditioned medium of DJ-1 knockout cells and in serum from DJ-1 knockout mice using Western blotting and ELISA. We performed immunocytochemical staining of legumain to examine the localization of legumain in DJ-1-knockout cells.Results:We found that the secretion levels of legumain were increased in the conditioned medium of DJ-1-knockout cells and in serum from DJ-1-knockout mice. Dot structures of legumain were also increased in DJ-1-knockout cells.Conclusion:The results suggest that legumain secretion from DJ-1-knockout cells and in mice increases through its increased expression and accumulation in membrane-associated vesicles.
Highlights
Asparaginyl endopeptidase, known as legumain (EC 3.4.22.34) belongs to the cysteine peptidase C13 family [1]
We found that the secretion levels of legumain were increased in the conditioned medium of DJ-1-knockout cells and in serum from DJ-1-knockout mice
The results suggest that legumain secretion from DJ-1-knockout cells and in mice increases through its increased expression and accumulation in membrane-associated vesicles
Summary
Asparaginyl endopeptidase, known as legumain (EC 3.4.22.34) belongs to the cysteine peptidase C13 family [1]. We found that transcription of the legumain gene is regulated by p53 [11] through DJ-1 [12]. We found that legumain secretion into serum was increased in DJ-1- knockout mice, that legumain localization was altered in DJ-1-knockout cells compared to that in wild-type cells, and that legumain concentration in membrane-associated vesicles was increased in DJ-1-knockout cells. These results suggest that prolegumain secretion increases in DJ-1-knockout mice through its accumulation in membrane- associated vesicles. Asparaginyl endopeptidase, known as legumain (EC 3.4.22.34) shows strong activity in the mouse kidney. We reported that transcription of the legumain gene is regulated by p53 through DJ-1
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