Secretion of IgM antibodies by glass-adherent fraction of immune mouse spleen cells: I. Immunological activity of glass-adherent cells

Abstract

High specific plaque-forming activity was associated with the firmly adherent fraction of mouse spleen cells immunized with SRC (3–8% of the total spleen population). The specific activity of the adherent fraction increased 7–8 times when the adherence occurred in presence of 1% BSA. The plaque-forming capacity of glass-adherent cells was quantitatively inhibited by anti IgM serum, by actinomycin D, and by puromycin. The rates of inhibition by actinomycin D and by puromycin were similar to the rate of inhibition of the nonfractionated population. The formation of passive plaques could not be achieved with the adherent cell fractions, nor with the nonfractionated spleen cells, under the conditions which produced passive rosettes. Experimental evidence is submitted, indicating that there is active IgM antibody secretion by the cells attached to glass.