Abstract

Summary Corneal epithelium from embryonic chicken was isolated in organ culture on various nonliving substrata in the presence of 3 H-proline. Autoradiographs showed that the epithelium secreted into the substrata, radioactive material resistant to extraction by sodium hydroxide. In certain explants it was possible to demonstrate by electron microscopy that the radioactivity was associated with striated fibrils and sheets that had the repeat period and interband pattern typical of native collagen. A newly formed basement lamina was also seen.

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