Abstract
The synthesis and secretion of progesterone- 14C and pregn-4-ene-2α-ol-3-one-14C in vivo has been demonstrated in the rat with luteinized ovaries by retrograde infusion of cholesterol-4- 14C solubilized by incubation with serum prior to administration into the vena cava. “Solubilized” cholesterol-4- 14C derived from blood circulation can penetrate to intracellular sites in ovaries and rapidly label the endogenous active cholesterol pool(s) for steroidogenesis. Radioactive progestins formed in the ovaries are released into the circulating blood within minutes. An increase both in mass and radioactivity of secreted progestins following exogenous LH or NADPH administration was found. There was a much more rapid effect on steroidogenesis by NADPH infusion than that observed after LH administration. A higher specific activity of secreted progestins was obtained following stimulation by NADPH, whereas the specific activity after LH stimulation was the same as found in controls. The modes of action of LH and NADPH on steroidogenesis invivo are discussed.
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