SECRETION AND PROLIFERATIVE EFFECTS OF CHICKEN GROWTH HORMONE IN TESTICULAR CELL CULTURES TREATED WITH GHRH

Abstract

Event Abstract Back to Event SECRETION AND PROLIFERATIVE EFFECTS OF CHICKEN GROWTH HORMONE IN TESTICULAR CELL CULTURES TREATED WITH GHRH Carlos G. Martínez-Moreno1, Luz M. López-Marín2, Martha E. Carranza1, Carlos Arámburo1 and Maricela Luna1* 1 Universidad Nacional Autonoma de Mexico, Institute of Neurobiology, Dept. of Molecular and Cellular Neurobiology, Mexico 2 Universidad Nacional Autónoma de México, Centro de Física Aplicada y Tecnología Avanzada, Mexico Recent findings showed the cytoplasmic and nuclear distribution of growth hormone (GH) and its receptor (GHR) in chicken testicular germ cells, suggesting an intracrine, autocrine and/or paracrine role for this hormone during spermatogenesis. The expression of testicular GH may be modulated by a variety of factors, including growth hormone releasing hormone (GHRH) and Pit-1. We analyzed the effect of GHRH (1, 10 and 100 nM, for 2 h) on GH release into the media of primary cell cultures derived from chicken testes at two developmental stages: during puberty (20 weeks, PC) and sexual maturity (32 weeks, MC). Released GH increased after GHRH treatment both in PC media (10.8±1, 13.4±2 and 14.5±2 ng/ml) and MC media (5.83±2.05, 7.6±0.6 and 8.5±2 ng/ml) in comparison to the corresponding controls (7.4±1 and 3.2±0.7 ng/ml, respectively). Western blot analyses were performed to characterize whether GHRH modulation involved secretion of particular GH isoforms. Interestingly, 15 kDa and 26 kDa GH variants were differentially modulated after 100 nM GHRH treatment, resulting in a 2.5 fold-increase in 15 kDa GH secretion, whereas a significant decrease was found for the 26 kDa variant release. On the other hand, GHRH (1, 10, 100 nM) stimulated proliferation of testicular cells in culture (2.7-, 3.6- and 2.0-fold, respectively, in comparison to the controls) as determined by the PCNA assay. Using the 3H-thymidine incorporation assay GH also increased the proliferation of testicular cells (by 118.7±18.1%, and 170.8±28%, and 102.1±15.7% at 1, 10 and 100 nM, respectively, when compared to 96.1±12.4% in the untreated controls). Altogether, these results suggest a role of GHRH and GH during chicken testicular cell proliferation, probably through autocrine/paracrine mechanisms. Acknowledgements This work was supported by grants CONACyT (F1-60296) and PAPIIT-UNAM (IN-210209), graduate fellowships from CONACyT (185024) and PDCB-UNAM and Alejandro Bayón Fund established by CA Keywords: autocrine/paracrine mechanisms, extrapituitary growth hormone, GHRH, Growth Hormone, proliferation, testicular cell cultures, testicular growth hormone variants Conference: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011. Presentation Type: Poster Topic: Gonadal development and gamete maturation Citation: Martínez-Moreno CG, López-Marín LM, Carranza ME, Arámburo C and Luna M (2011). SECRETION AND PROLIFERATIVE EFFECTS OF CHICKEN GROWTH HORMONE IN TESTICULAR CELL CULTURES TREATED WITH GHRH. Front. Endocrinol. Conference Abstract: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology. doi: 10.3389/conf.fendo.2011.04.00094 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Dr. Maricela Luna, Universidad Nacional Autonoma de Mexico, Institute of Neurobiology, Dept. of Molecular and Cellular Neurobiology, Queretaro, Queretaro, 76230, Mexico, lunam@servidor.unam.mx Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers Carlos G Martínez-Moreno Luz M López-Marín Martha E Carranza Carlos Arámburo Maricela Luna Google Carlos G Martínez-Moreno Luz M López-Marín Martha E Carranza Carlos Arámburo Maricela Luna Google Scholar Carlos G Martínez-Moreno Luz M López-Marín Martha E Carranza Carlos Arámburo Maricela Luna PubMed Carlos G Martínez-Moreno Luz M López-Marín Martha E Carranza Carlos Arámburo Maricela Luna Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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