Abstract
Gastrin has been established as an important trophic hormone for certain tissues of the gastrointestinal tract. This study examined the trophic response of the gastrointestinal tract to pentagastrin, secretin, metiamide (antihistamine, H2 receptor blocker), and histamine by examining their effects on mucosal DNA synthesis. Fasted rats were killed 16 hr after an injection of saline, pentagastrin (250 ?tg per kg), metiamide (2 mg per kg), secretin (75 U per kg), histamine (20 mg per kg), or combinations of secretin and pentagastrin, or metiamide and pentagastrin. Pieces of tissue from the oxyntic gland region, duodenum, and ileum, were incubated for 30 min with 2 gc per ml of [3H]thymidine. DNA was extracted, and the incorporation of thymidine was determined by liquid scintillation counting. Results were expressed as disintegrations per minute per milligram of wet weight of tissue, or per microgram of DNA as a percentage of the saline-injected control animals. There was no significant difference between these two means of expression. Pentagastrin stimulated DNA synthesis to 50, 60, and 100% (P
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