Abstract
BackgroundThe Wnt signaling pathway is abnormally activated in many human cancers. Secreted frizzled-related proteins (SFRPs) function as negative regulators of Wnt signaling and play an important role in carcinogenesis. SFRP promoter hypermethylation has often been identified in human cancers; however, the precise role of SFRPs in cutaneous squamous cell carcinoma (SCC) is unclear.MethodsThe methylation status of the SFRP family was analyzed in an age-and sex-matched case-control study, including 40 cutaneous SCC cases and 40 normal controls, using the MassARRAY EpiTYPER system.ResultsThe methylation rate of SFRP1, SFRP2, SFRP4, and SFRP5 promoters was significantly higher in cutaneous SCC tissues than in adjacent tissue and normal skin samples.DiscussionOur manuscript mainly discussed the average methylation rate of SFRPs (SFRP1, SFRP2, SFRP4, and SFRP5) promoters are significantly high in tumor tissue samples and the average CpG island methylation rate among different pathological levels of cutaneous SCC between these genes are different.ConclusionsOur findings suggest that promoter hypermethylation of SFRPs is associated with the development of carcinoma, and could be a useful tumor marker for cutaneous SCC and other types of cancers.
Highlights
The Wnt signaling pathway is abnormally activated in many human cancers
Our findings suggest that promoter hypermethylation of Secreted frizzled-related proteins (SFRPs) is associated with the development of carcinoma, and could be a useful tumor marker for cutaneous squamous cell carcinoma (SCC) and other types of cancers
We investigated the promoter methylation rate of SFRP1, SFRP2, SFRP4, and SFRP5 in 40 cutaneous SCC tissues, 40 adjacent tissues, and 40 normal samples using the MassARRAY EpiTYPER system
Summary
Secreted frizzled-related proteins (SFRPs) function as negative regulators of Wnt signaling and play an important role in carcinogenesis. SFRP promoter hypermethylation has often been identified in human cancers; the precise role of SFRPs in cutaneous squamous cell carcinoma (SCC) is unclear. Non-melanoma skin carcinomas, comprising cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC), are the most common malignancies in fair-skinned Caucasians [1]. The Wnt family of proteins includes a wide range of secreted growth factors that regulate cell differentiation, the involvement of Wnt signaling in carcinogenesis has been extensively studied, the associations of the SFRP family with tumorigenesis have only recently begun to be explored. Wnt5a signaling was found to contribute to tissue invasion by non-melanoma skin cancer, including both SCC
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