Abstract
Water-soluble luminescent colloidal quantum dots (QDs) have attracted much attention in biological and medical applications as drug carriers and imaging agents for cells. Investigations of protein–nanoparticle interaction play a key role in biomedical application because when nanoparticles enter the cellular environment, they strongly interact with the intra-cellular protein that may transmit biological signals due to alter protein conformation. The perturbed signal transduction in cells may cause toxicity. Herein, we report on the interaction of semiconductor quantum dots (CdSe) with proteins-bovine serum albumin (BSA), -lactoglobulin ( -Lg) and Human Serum Albumin (HSA). The interactions have been studied by UV-Vis spectroscopy, surface tension measurements, Circular Dichroism (CD) and Raman studies. Protein molecules were adsorbed on the surface of colloidal CdSe through the capping agent 3-mercaptopropionic acid (MPA) making these hydrophilic. Tensiometry and UVabsorbance data showed a finite protein-QDs interaction at different QD concentration. Results from CD and Raman studies suggest that the proteins underwent substantial conformational and hydration changes at the secondary structure level.
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