Abstract

Dynamic light-scattering (DLS), fluorescence spectroscopy (FS) and circular dichroism (CD) techniques were applied to study the influence of alcohol on beta-casein (b-CN) self-association and the secondary structure in a wide range of temperatures and ethanol concentrations. Temperature induced micellization and demicellization transitions of b-CN in water-ethanol solutions are revealed on the basis of the DLS data. The obtained results indicate that the association of b-CN at low and high alcohol concentrations proceeds through different mechanisms. It is suggested that the solvent microheterogeneity independently modulates both the secondary structure and the colloid properties of b-CN.

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