Secondary screening of strains of antagonists to a Phoma pathogen on sunflower

Lyubov Maslienko,Daria Punogina,Lyubov Datsenko,Evgeniya Efimtseva,Aliya Voronkova

doi:10.1051/bioconf/20202100017

Lyubov Maslienko, Daria Punogina + Show 3 more

Open Access

https://doi.org/10.1051/bioconf/20202100017

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Abstract

There are presented data of the first stage of the secondary screening of antagonist strains from a collection of the biological methods laboratory of V.S. Pustovoit All-Russian Research Institute of Oil Crops to the aggressive isolate of Phoma macdonaldii Boerema infecting sunflower. We used methods of ‘agar blocks’ and ‘perforated Petri dishes’. As a result of a screening of 55 antagonist strains to Phoma macdonaldii pathogen we selected 17 promising strains-producers of microbiological preparations having biological efficiency exceeding 40.0 %. In control variant without seed treatment sunflower seedlings infection was up to 76.2 %, and maximal biological efficiency of laboratory samples from fungi-producers were determined for a strain M-24 Penicillium sp. (73.8 %); from bacteria of a genus Bacillus – for a strain D-10 Bacillus sp. (74.2%); from bacteria of a genus Pseudomonas – for a strain Sgc-1 Pseudomonas sp. (73.8 %). Maximal colonizing activity were noted for fungi-producers M-24 Penicillium sp. (85.7 %) and Xk-1 Ch. olivaceum (71.4 %), as well as for bacteria of a genus Bacillus – 5B-1 B. subtilis (71.4 %) and a genus Pseudomonas – Oif 2-1 Pseudomonas sp. and 14-3 P. chlororaphis (71.4 %) against Phoma infection in control of 70.0 %.

Highlights

There are presented data of the first stage of the secondary screening of antagonist strains from a collection of the biological methods laboratory of V.S
To conduct the first stage of the secondary screening based on the promising strains, we accumulated laboratory samples of microbiological preparations in a preparative form ‘liquid culture’ (LC)
As a result of the evaluation of the laboratory samples of the microbiological preparations in the preparative form of LC based on 55 strains of fungi and bacteria-antagonists isolated in the initial screening, we selected 17 promising strains-producer that showed biological efficacy excessing 40.0% in conditions of high artificial infection of sunflower with Phoma macdonaldii in the laboratory using the “agar block” method

Summary

Introduction

There are presented data of the first stage of the secondary screening of antagonist strains from a collection of the biological methods laboratory of V.S. Pustovoit All-Russian Research Institute of Oil Crops to the aggressive isolate of Phoma macdonaldii Boerema infecting sunflower. As a base of a worked out by us conception of task-oriented development of microbiological preparations for protection of sunflower and other agricultural crops against diseases we use a search of antagonist strains which are safety for people, non-phytotoxic, possessing high activity in widely varying environments against a complex of pathogens, being of polyfunctional action. As a result of long-term researches we have created a collection of promising strains of fungi and bacteria-antagonists to a wide range of pathogens infecting oil and other agricultural crops. We did not find in the literature information on the development of biological protection measures against the Phoma rot on sunflower, except for testing biological preparations against the Phoma rot on tea [17]

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