Abstract
Yearling rainbow trout were immunized by intraperitoneal injection of 1 mg dinitrophenol-keyhole limpet haemocyanin (DNP-KLH) or 10 9 formalin-inactivated Yersinia ruckeri. Fish were regularly bled to establish individual kinetics by passive haemagglutination and agglutination, respectively. Groups of 20 to 30 fish were used. A control group was also provided which received its first injection simultaneously with the booster injection for the experimental group. The secondary anti-DNP response was never found to be enhanced when compared with the primary one but, in contrast, a noticeable secondary response was observed with the bacterial antigen.
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