Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria Amycolatopsis taiwanensis.

Yung-Shun Su,Ming-Der Wu,Chee-Yin Chai,Aij-Lie Kwan,Ming-Jen Cheng,Jih-Jung Chen,Yueh-Hsiung Kuo

doi:10.3390/molecules26195765

Abstract

Phytochemical investigation and chromatographic separation of extracts from one new actinobacteria strain Amycolatopsis taiwanensis that was isolated from soil of Yilan township, in the north of Taiwan, led to the isolation of nine new compounds, amycolataiwanensins A–I (1–9, resp.), and one new natural product, namely amycolataiwanensin J (10). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic-data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Of the isolates, 3, 5, 7 and 8 exhibited potent anti-NO production activity, with IC50 values of 17.52, 12.31, 17.81 and 13.32 μM, respectively, compared to that of quercetin, an iNOS inhibitor with an IC50 value of 35.94 μM. This is the first report on indole metabolite from the genus Amycolatopsis.

Highlights

IntroductionActinobacteria are well known as an outstanding and fascinating source of commercially valuable bioactive compounds, antibiotics
The following instruments were used for obtaining physical and spectroscopic data: optical rotations Jasco DIP-370 polarimeter; in CHCl3 (JASCO, Kyoto, Japan); FTIR spectra were obtained by using a FTIR spectrometer (Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1, Norwalk, CT, USA); Absorption spectra were recorded by an ultraviolet visible (UV-vis) light spectrophotometer (Jasco UV-240 spectrophotometer; λmax in nm, Hitachi, Ltd., Tokyo, Japan), EI and HREIMS: Jeol JMS-HX-300 mass spectrometer; in m/z and JEOL SX-102A Mass Spectrometer; melting point, MP-J3 (Yanaco, Kyoto, Japan); NMR spectra were taken on a Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers with TMS as an internal standard (Lake Forest, California USA)
Actinobacteria have the ability to produce a variety of physiologically active products, so they play a very important role in the food and pharmaceutical industries

Summary

Introduction

Actinobacteria are well known as an outstanding and fascinating source of commercially valuable bioactive compounds, antibiotics. Almost a half of the known microbial bioactive secondary metabolites are derived from actinomycetes, of which more than 70% were obtained from the genus Streptomyces. They seem to have unlimited ability to produce secondary metabolites with multiple chemical structures and biological activities, so they have a place in the pharmaceutical industry [1,2,3,4,5].

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Conclusion