Abstract
Objective: This study aimed to isolate secondary metabolites from the aerial parts of endemic Centaurea aphrodisea Boiss. using several chromatographic methods and elucidate the structure of the compounds by using spectroscopic methods. Material and Method: Aerial parts of the endemic C. aphrodisea were collected from Bozdağ (Ödemiş, İzmir) and n-hexane, chloroform and metanol extracts were prepared. The chloroform extract was investigated by using various chromatographic methods, and the structures of the isolated compounds were determined using spectroscopic methods (1D-2D NMR and LC-MS). Result and Discussion: One elemane type sesquiterpene (methyl 8α,6α,15-trihydroxyelema-1,3,11(13)-trien-12-oate) and four flavone derivatives (cirsimaritin, 3'-O-methyl eupatorin, eupatorin and salvigenin) were isolated and identified. In addition, the presence of a phenylpropanoid glycoside (syringin) was determined in a fraction by comparison with a referance compound using TLC technique. These compounds are reported for the first time from C. aphrodisea with this study.
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