Secondary metabolites and anti-microbial/anti-oxidant profiles in Ocimum spp.: Role of soil physico-chemical characteristics as eliciting factors

Abstract

Ocimum has long been used as a medicinal plant, although little information is available about its bioactive ingredients, and the influence of soil properties on modulation of secondary metabolites in Ocimum has yet to be ascertained. In this study, we present a thorough survey of all potential metabolic compounds in O. sanctum and O. basilicum. In both species, certain compounds (e.g., quercetin, kaempferol, catechin, and S-adenosyl homocysteine) were detected coincidently. In the case of O. basilicum, other vital phenolic acids (e.g., ursolic, vanilic, coumaric, and syringic acids) were identified. The aqueous extracts (AEs) of Ocimum recorded decrease of 6–94% in the proliferation of pathogenic bacteria (e.g., Listeria monocytogenes, Staphylococcus sp., Salmonella sp., and Bacillus sp.). The AEs also showed effective antioxidant activity by reducing free radicals by a factor of 1.04–1.13. Root-zone soil samples of both Ocimum spp. were collected from strategic locations with varying levels of key soil attributes (e.g., soil organic carbon (SOC), microbial biomass carbon (MBC), urease, and phosphatase). At high levels of SOC, MBC, and soil enzymes, the bioactivity of Ocimum spp. was observed to be promoted, especially with respect to secondary metabolite expression, anti-pathogenic activity, and anti-oxidant properties. As such, the findings of strong correlations between secondary metabolite concentrations and bioactivity attributes in Ocimum suggest the potent role of soil quality in eliciting the production of secondary metabolite in association with bioactivity in Ocimum spp.