Secondary metabolite Constituents, Antimicrobial Activity and Gas chromatography-Mass spectroscopy Profile of Bombax buonopozense P. Beauv.(Bombacaceae) Stem bark Extract

Abstract

This study evaluated the phytochemical constituents, antimicrobial activity and structural characteristics using GC-MS of bioactive constituents from the stem bark extract of Bombax buonopozense. Secondary metabolite constituents was assayed using standard procedures. The antimicrobial activity was carried out using agar diffusion method to determine the susceptibility of these microorganisms: (Staphylococcus aureus (ATCC 29213), Streptococcus faecials, Bacillus subtilis (ATCC 8263), Escherichia coli (ATCC 25922), Salmonella tiphi, Pseudomonas aeruginosa (ATCC 27853), Candida albican (ATCC 90029), Aspergillusniger and Phytophera megakarya. EtOAc fraction was chromatographed on silica column to afford oil isolate and its chemical structures were proven with the employment of GC-MS. Phytochemical screening revealed the presence of saponins, flavonoids, reducing sugars, steroids, terpenoids, anthraquinone, phenols and alkaloids. The antimicrobial sensitivity test showed higher activity on Candida albican, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus, moderate to little activity on the rest organisms. The GC-MS of the stem bark oil extract revealed the main constituents as: 1, 2 Benzenedicarboxylic acid 4.10%, Diisobutyl phthalate 1.18%, Dibutyl phthalate 0.72%, Lupenone 0.62%, Lupeol 4.93%, Lupenyl acetate 86.28%, Testosterone decanoate 0.87% and 3-O-Acetyl-6-methylcycloartenol 0.67%. The findings indicate stem bark extract has bioactive compounds which may be effective in the treatment of infection diseases.