Abstract
The plaque-forming cell (PFC) response of spleen cells from mice primed 30-90 days previously with chicken y globulin (CGG) plus adjuvant was studied after restimulation in vitro with 1 pg to 100 μg CGG per culture. The peak response (100 to 500 PFC per 106 cells), elicited with 0.1 μg CGG, was detected five days after restimulation. The 100 μg level of CGG suppressed the PFC response to CGG, but did not decrease the primary PFC response to sheep red blood cells (SRBC) of other, control cultures. Plaques detected with CGG coupled immunologically to SRBC were clearer but not more numerous than those detected with CGG coupled chemically to SRBC. The system described permits the investigation of cellular mechanisms involved in the antibody response to a soluble protein antigen and is, therefore, ideally suited to elucidation of factors which enhance or suppress antibody biosynthesis and immunologic memory.
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