Abstract
A fluorometer has been developed which enables the rapid collection of two-photon excitation spectra at submillimolar levels. The principle of operation involves sinusoidal modulation of a cavity-dumped dye laser excitation pulse train, followed by lock-in detection of the second harmonic signal induced by nonlinear absorption. Because of the intrinsic ability to reject interferences which vary linearly with incident laser power, signal processing is 4 to 5 times faster than standard regression methods
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