Second Derivative Spectrophotometric Determination of Iron(II) and Ruthenium(III) Using 1, 10-phenanthroline

Abstract

A simple and sensitive derivative spectrophotometric method has been developed for the determination of Fe(II) and Ru(III) using the baseline to peak measurement techniques. In the present method 1,10-phenanthroline (o-phen) used as a complexing agent. Fe(II) and Ru(III) forms a complex with o-phen at pH 5 and 4, respectively. Based on baseline-to-peak measurement techniques deep red Fe(II)-o-phen complex shows the maximum peak at 535 nm, while the yellow product of Ru(II) - o-phen complex at 466 nm. Beer's law obeyed in the range of 0.1 - 2.0 μg mL-1 for Fe(II) and 5.0 - 20.0 μg mL-1 for Ru(III) which is supported by correlation coefficient 0.99853 and 0.99914, respectively. The present method has been applied successfully for the determination of Fe (II) in pharmaceutical formulations and Ru(III) in some synthetic mixtures.