Seasonal Vitamin A Depletion in Grazing Horses Is Assessed Better by the Relative Dose Response Test than by Serum Retinol Concentration

Abstract

Vitamin A influences growth and reproduction in horses. A retinol dose response (RDR) test for retinol has been shown to be better than serum retinol concentration for assessing vitamin A status in other species, so we have compared these two methods in the horse. Forty-five Thoroughbred broodmares were assigned randomly to three groups fed pasture and hay (PH), pasture, hay and vitamin A-free concentrate (PHC), or hay and concentrate (HC) in early summer (May 1991). Mares in pasture groups produced 23 foals (March through June) that had access to their dam's diets and were also studied. In the mares, significant vitamin A depletion developed in 2 mo in the nonpasture group (HC) and in 8 mo in the two pasture groups (PH and PHC) according to the RDR test, and in all three groups at 8 mo as shown by a decrease in serum retinol concentration. In the weanlings (PH and PHC only), no differences between groups were found for serum retinol, but the RDR was significantly higher in the PH group, which had suffered a respiratory infection, than in the PHC group. These findings indicated that vitamin A depletion was detected more readily by the RDR test than by serum retinol concentration, that consumption of pasture delayed depletion in the late fall, and that infection was associated with lower vitamin A status.