Seasonal variation of temperature affects HMW-PAH accumulation in fishery species by bacterially mediated LMW-PAH degradation

Abstract

Currently, the specific mechanism generating seasonal variation in polycyclic aromatic hydrocarbons (PAHs) via bacterial biodegradation remains unclear, and whether this alteration affects PAH bioaccumulation is unknown. Therefore, we performed a study between 2015 and 2020 to investigate the effects of seasonal variation on bacterial communities and PAH bioaccumulation in the Pearl River Estuary. Significantly high PAH concentrations in both aquatic and fishery species were determined in dry seasons (the mean ∑16PAH concentration: water, 37.24 ng/L (2015), 30.83 ng/L (2020); fish, 51.01 ng/L (2015) and 72.60 ng/L (2020)) compared to wet seasons (the mean ∑16PAH concentration: water, 22.38 ng/L (2015), 19.40 ng/L(2020); fish, 25.28 ng/L (2015) and 32.59 ng/L (2020)). Distinct differences in taxonomic and functional composition of bacterial communities related to biodegradation of low molecular weight PAHs (LMW-PAHs) were observed between seasons, and the concentrations of PAHs were negatively correlated with seasonal variation in temperature. Temperature-related specific bacterial taxa (e.g., Stenotrophomonas) directly or indirectly participated in LMW-PAH degradation via encoding PAH degradation enzymes (e.g., protocatechuate 4,5-dioxygenase) that subsequently led to bioaccumulation of high molecular weight PAHs (HMW-PAHs) in wild and fishery species due to LMW-PAHs in the water. Based on this alteration, the ecological risk posed by PAHs decreased in wet seasons, and an unbalanced spatio-temporal distribution of PAHs was observed in this estuary. These results suggest that seasonal variation of temperature affects HMW-PAH accumulation in fishery species via bacterially mediated LMW-PAH biodegradation.