Abstract
The seeds of four Eucalyptus species (Origin: Australia) named, Eucalyptus intertexta var. intertexta R.T. Baker, Eucalyptus platypus var. heterophylla Blakely, Eucalyptus leucoxylon var. rosea Miq. and Eucalyptus camaldulensis var. obtuse Blakely were cultivated in the years 1993–1994 in Kashan in Isfahan province (Central regions of Iran). These species have good adaptability with the climatic condition of this area (dry and warm weather). The leaves of these species were collected in the middle of four seasons (spring, summer, autumn and winter), for finding the best harvesting time for obtaining the highest oil yield and 1,8-cineole content. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. Analysis of variance showed harvesting time had significant effect on the oil yields and 1,8-cineole content of these Eucalyptus species. Nineteen components were identified in the oils of Eucalyptus intertexta with 1,8-cineole (67.2–83.1 %) and globulol (1.9–8.9 %) as main constituents. The highest percentage of essential oil and 1,8-cineole were found in summer and winter, respectively. Twenty-three compounds were characterized in the oil of Eucalyptus platypus with 1,8-cineole (27.1–56.4 %) and α-pinene (7.3–31.7 %) as main constituents. The highest percentage of essential oil and 1,8-cineole was found in autumn. Fourteen compounds were identified in the oil of Eucalyptus leucoxylon with 1,8cineole (41.7–85.5 %) as main constituent. The highest percentage of essential oil and 1,8-cineole was found in spring. Twenty-nine components were identified in the oils of Eucalyptus camaldulensis with 1,8-cineole (26.1–51.6 %) and a-pinene (7.6–13.2 %) as main constituents. The highestpercentage of essential oil and 1,8-cineole was found in summer and autumn, respevtively. The results showed, with considering the highest oil yield and 1,8-cineole content together, the best harvesting time for E. intertexta and E. platypus was autumn, E. leucoxylon was spring and E. camaldulensis was summer.
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