Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (Citellus dauricus Brandt).

Abstract

Sperm-associated antigen 11A (SPAG11A), a protein expressed exclusively in the epididymis, plays a vital and special role in regulating mammalian sperm maturation. The aim of this study was to investigate the seasonal expressions of SPAG11A and androgen receptor (AR) in the epididymis of the wild ground squirrels (Spermophilus dauricus Brandt). Morphologically, the results showed that epididymis length and weight in the breeding season were significantly higher than those of the non-breeding season. Histologically, the results revealed that enlarged lumen diameters, thickened epithelium and abundant sperm in the breeding season while reduced lumen diameters and epithelium with no sperm in the non-breeding season. SPAG11A was intensely expressed in cytoplasm and nucleus of epithelial cells and smooth muscle cells in the breeding season, and weaker staining in the non-breeding season. In the lumen of epididymis, SPAG11A immunostaining in the sperm of the epididymal corpus and cauda was higher than those in the caput during the breeding season. The immunostaining of AR was only presented in nucleus of smooth muscle cells and epithelial cells in the epididymis from the breeding season rather than the non-breeding season. The results of real-time quantitative PCR also showed that the mRNA levels of SPAG11A and AR in the epididymis during the breeding season were significantly higher than those of the non-breeding season. In addition, the circulating testosterone, follicle- stimulating hormone and luteinizing hormone levels in the squirrels were higher in the breeding season compared with those in the non-breeding season. Taken together, these results implied that SPAG11A might be involved in regulating seasonal changes in epididymal functions of the wild ground squirrels.