Seasonal changes of fatty acid composition and thermotropic behavior of polar lipids from marine macrophytes

Abstract

Major glyco- and phospholipids as well as betaine lipid 1,2-diacylglycero- O-4′-( N,N,N-tri-methyl)-homoserine (DGTS) were isolated from five species of marine macrophytes harvested in the Sea of Japan in summer and winter at seawater temperatures of 20–23 and 3 °C, respectively. GC and DSC analysis of lipids revealed a common increase of ratio between n−3 and n−6 polyunsaturated fatty acids (PUFAs) of polar lipids from summer to winter despite their chemotaxonomically different fatty acid (FA) composition. Especially, high level of different n−3 PUFAs was observed in galactolipids in winter. However, the rise in FA unsaturation did not result in the lowering of peak maximum temperature of phase transition of photosynthetic lipids (glycolipids and phosphatidylglycerol (PG)) in contrast to non-photosynthetic ones [phosphatidylcholine (PC) and phosphatidylethanolamine (PE)]. Different thermotropic behavior of these lipid groups was accompanied by higher content of n−6 PUFAs from the sum of n−6 and n−3 PUFAs in PC and PE compared with glycolipids and PG in both seasons. Seasonal changes of DSC transitions and FA composition of DGTS studied for the first time were similar to PC and PE. Thermograms of all polar lipids were characterized by complex profiles and located in a wide temperature range between −130 and 80 °C, while the most evident phase separation occurred in PGs in both seasons. Polarizing microscopy combined with DSC has shown that the liquid crystal – isotropic melt transitions of polar lipids from marine macrophytes began from 10 to 30 °C mostly, which can cause the thermal sensitivity of plants to superoptimal temperatures in their environment.