Abstract
In a previous paper (1), it was shown that there were considerable variations in the quantity of insoluble nitrogen, both in bark and wood, during the growth cycle of pear shoots. It was also shown in another paper (2) that seasonal variations in the water-soluble nitrogen fraction in pear shoots are not only quantitative but qualitative also. It was of interest to find how the insoluble nitrogen fraction, representing mainly cytoplasmic proteins, behaved in this respect. As a convenient way of partially answering this question, a Van Slyke fractionation of the insoluble nitrogen was undertaken, although it was apparent that the constancy of results by this method would be no proof of the constant composition of this fraction.
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