Seasonal changes in humidity impact drought resistance in tropical Drosophila leontia: Testing developmental effects of thermal versus humidity changes

Abstract

Drosophila leontia is native to highly humid equatorial tropical habitats but its desiccation sensitivity (~10h) is not consistent with its abundance during the drier autumn season in the subtropical regions. We have tested the effects of developmental acclimation on desiccation resistance and water balance related traits of D. leontia collected during rainy and autumn seasons. The isofemale lines of seasonal populations were reared under ecologically relevant growth temperatures (18 or 26°C) or humidity conditions (35 or 85% RH) but tested at different times under identical experimental conditions. The larvae as well as flies reared under two thermal conditions (18 or 26°C) showed no effect on desiccation related traits as well as storage and utilization of energy metabolites. In contrast, for D. leontia reared under low humidity (35% RH), significant changes at larval as well adult stages include increase in the desiccation resistance as well as cuticular lipid quantity, reduced levels of rate of body water loss, higher storage of carbohydrates but lower rate of utilization of carbohydrates as compared with flies reared at high humidity (85% RH). D. leontia has responded to rearing under low humidity conditions by increasing its desiccation resistance but not due to changes in the growth temperatures. These laboratory observations on seasonal populations highlight differences due to rearing conditions but not due to seasons. Further, direct analysis of wild-caught seasonal populations has shown trends similar to developmental acclimation effects. For wild caught flies, there are significant seasonal differences i.e. higher desiccation resistance as well as cuticular lipid quantity but reduced rate of water loss for autumn than rainy season flies. Thus, our laboratory observations are relevant for understanding seasonal adaptations of natural populations of tropical D. leontia to wet-dry conditions in the wild.