Seasonal changes in expression of nerve growth factor and its receptors TrkA and p75 in the ovary of wild ground squirrel (Citellus dauricus Brandt)

Ben Li,Yuning Liu,Yingying Han,Kazuyoshi Taya,Shiyang Huang,Qinglin Li,Qiang Weng,Gen Watanabe,Xia Sheng,Lihong Bao

doi:10.1186/1757-2215-7-3

Abstract

The aim of this study was to investigate the presence of nerve growth factor (NGF) and its receptors tyrosine kinase A (TrkA) and p75 in the ovaries of the wild ground squirrels during the breeding and nonbreeding seasons. In the breeding period, NGF, TrkA and p75 were immunolocalized in granulosa cells, thecal cells, interstitial cells and luteal cells whereas in the nonbreeding period, both of them were detected only in granulosa cells, thecal cells and interstitial cells. Stronger immunostaining of NGF, TrkA and p75 were observed in granulosa cells, thecal cells and interstitial cells in the breeding season compared to the nonbreeding season. Corresponding for the immunohistochemical results, immunoreactivities of NGF and its two receptors were greater in the ovaries of the breeding season then decreased to a relatively low level in the nonbreeding season. The mean mRNA levels of NGF, TrkA and p75 were significantly higher in the breeding season than in the nonbreeding season. In addition, plasma gonadotropins, estradiol-17β and progesterone concentrations were significantly higher in the breeding season than in the nonbreeding season, suggesting that the expression patterns of NGF, and TrkA and p75 were correlated with changes in plasma gonadotropins, estradiol-17β and progesterone concentrations. These results indicated that NGF and its receptors, TrkA and p75 may be involved in the regulation of seasonal changes in the ovarian functions of the wild ground squirrel.

Highlights

The nerve growth factor (NGF) belongs to a family of related proteins required for the survival, maintenance, and development of discrete neuronal populations in the central and peripheral nervous systems [1,2,3]
The present study demonstrated that immunoreactivities of NGF and its two receptors, tyrosine kinase A (TrkA) and p75 were greater in the ovaries of the breeding season decreased to a relatively low level in the nonbreeding season, and the expression patterns of NGF ligand and receptors were correlated with the changes of plasma concentrations of gonadotropins, estradiol-17β and progesterone
These findings suggested that NGF, TrkA and p75 may be involved in the regulation of seasonal changes in the ovarian functions of the wild ground squirrels

Summary

Introduction

The nerve growth factor (NGF) belongs to a family of related proteins required for the survival, maintenance, and development of discrete neuronal populations in the central and peripheral nervous systems [1,2,3]. In our previous studies of the golden hamsters, NGF and its two receptors TrkA and p75 were present in ovaries, oviducts and uteri, demonstrating that NGF, TrkA and p75 have important autocrine and paracrine regulatory roles in the function of reproductive organs during the estrous cycle [16,25,26]. Data to support this concept in wild animals, is very limited. To study the basic mechanisms of NGF regulation of ovarian function during the breeding and nonbreeding seasons within the annual reproductive cycle, the wild ground squirrel offers a useful model without any manipulations

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Conclusion