Seasonal and spatial variations in the chemical components and the cellular effects of particulate matter collected in Northern China

Abstract

The health effects of airborne particulate matter (PM) are likely to be strongly influenced by its components. The relationship between the composition of PM and its biological effects has been investigated in vitro/vivo, but more studies are needed to achieve a better understanding of the relationship. Such studies are limited in Northern China, where severe air pollution causes significant health impacts. In this study, we analyzed seasonal PM10 (PM, aerodynamic diameter less than 10μm) samples from five typical cities in Northern China for their physicochemical properties and their in vitro effects on A549 (human lung epithelial cell line) and RAW264.7 (murine monocyte macrophage) cells, including cytotoxicity, oxidative stress and inflammatory effects. Principal component analysis and multiple linear regressions were used to investigate the relationship between the PM components and the cellular responses. The cellular responses of A549 cells were more closely related to the endotoxin content and the levels of polycyclic aromatic hydrocarbons (PAHs) and their derivatives, while the cellular responses of RAW264.7 cells were largely related to PM10-bound metals, and the chalcophile elements (Pb, Cu, Zn, Cd) were more related to the PM-induced oxidative stress, whereas the lithophile and siderophile elements (Al, Fe, Mg, Co, V, Mn, Ca) were more related to PM-induced inflammation and cytotoxicity. As PM compositions changed seasonally, more intense cellular responses were seen when A549 cells were exposed to winter samples that contained higher levels of those components. The autumn and winter samples induced higher levels of oxidative stress in RAW264.7 cells, possibly due to higher contents of chalcophile elements, whereas the spring and/or summer samples were more cytotoxic and proinflammatory, possibly due to higher contents of lithophile and siderophile elements. The study suggests that the evaluation of health impacts induced by air pollution should take into account different physiochemical properties other than the mass concentration, and that public health would benefit greatly from effective, prioritized control of the sources that are the major producers of the central species.