Abstract
The aim of the study was to investigate specific potential markers for cells obtained from three layers of human AAA divided into three segments along the AAA based on morphological differences. The isolated cells were compared to control commercial cell types from healthy human abdominal aortas. For each type of aortic layer, three specimens from 6 patients were compared. Total RNA was isolated from 36 cell cultures for gene expression profiling and potential new cytometry markers were typed. Isolated cells were analyzed by flow cytometry by using fluorochrome-conjugated antibodies to markers: CNN1, MYH10, ENG, ICAM2, and TEK. The relative expression of 45 genes in primary cell cultures and control lines was analyzed. Statistically significant differences were found in the expression of most of the analyzed genes between individual layers and control lines. Based on relative expression, antibodies were selected for flow cytometry. Gene expression profiles allowed to select new potential cytometry markers: CNN1, MYH10, MYOCD, ENG, ICAM2, TEK. However, none of the tested markers seems to be optimal and characteristic for a specific layer of AAA.
Highlights
An abdominal aortic aneurysm (AAA) is a pathological dilatation of the infrarenal aorta
We analyzed the relative expression of marker genes in cultured cells assumed as standard cells (HAEC for endothelial cells in the internal layer—Internal layer (IL); Aortic Smooth Muscle Cells (AoSMC) for smooth muscle cells in middle layer—Middle layer (ML); and Aortic Adventitial Fibroblasts (AoAF) for fibroblasts in the external layer External layer (EL))
We focused on the ALCAM, CD40, CNN1, KRT18, KRT8, MYH10, MYOCD, ENG, TEK, CD34, CD70, CD90, CDH5, IL1R2, PECAM1, and S100A4 (Supplement Tables 2, 3, 4, 5)
Summary
An abdominal aortic aneurysm (AAA) is a pathological dilatation of the infrarenal aorta. It develops as a result of abdominal aortic wall dilation, which might lead to blood vessel rupture very often causing the patient’s death (Davis et al 2015). AAA is a multifactorial disease and many risk factors which can lead to AAA development have been identified. An abdominal aortic aneurysm is the cause of death in adults as a result of aortic rupture and the only treatment for Communicated by Michal Witt. Because no drug therapy is available for AAA, it is essential to understand its pathogenesis and know the specific markers involved in the development of AAA
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