SEARCHING AND IN SILLICO CHARACTERIZATION OF STREPTOMYCES PHAGE ENDOLYSINS AND THEIR CATALYTIC DOMAINS

Darina Peterkova,Michaela Mancoš,Andrej Godány,Zuzana Šramková

doi:10.15414/jmbfs.2020.10.2.221-229

Darina Peterkova, Michaela Mancoš + Show 2 more

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https://doi.org/10.15414/jmbfs.2020.10.2.221-229

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Abstract

Endolysins, peptidoglycan hydrolases encoded by bacteriophages, degrade bacterial cell wall and are promising alternative to antibiotics. Howewer, the selection and bioengineering of endolysins needs previous bioinformatic characterization. This study focuses on endolysins encoded by viruses infecting Streptomyces spp., describes Streptomyces phages diversity and association. Set of 143 endolysins were predicted in 175 Streptomyces phage genomes from NCBI database and The Actinobacteriophage Database and were characterized their phylogeny and function domains, especially catalytic domains. Predicted endolysins have amidase (Ami_2 and CHAP domain), muramidase (glycol_hydro_25) and peptidase activity (CHAP, NlpC/P60). This bioinformatic characterization serve as a base to next research in developing of endolysins with new properties in enzybiotik form.

Highlights

Phages or bacteriophages are specialized viruses able to infect host species belonging to the domain Bacteria
CHAP domain), muramidase and peptidase activity (CHAP, NlpC/P60). This bioinformatic characterization serve as a base to research in developing of endolysins with new properties in enzybiotik form
Endolysins are attractive for other fields, for example, in biotechnology for primary industry (Hoopes et al, 2009; Schmelcher et al, 2015); in food industry as the prevention against contamination by foodborne bacterial pathogens (Schmelcher and Loessner, 2016); and as a diagnostic tool to detect bacteria in different types of samples (Kretzer et al, 2007; Bai et al, 2016; Gómez-Torres et al, 2018)

Summary

Introduction

Phages or bacteriophages are specialized viruses able to infect host species belonging to the domain Bacteria. The traditional phage therapy is associated with some risks such as adaptation of viable viruses to target other, closely related strains, transduction of virulence genes, lysogenic conversion of commensal bacteria and bacterial resistance to phages (Valero-Rello, 2019). For this reason, not the whole phage particles but products of their genes, lytic enzymes such as endolysins, represent more useful tool against multiresistant bacteria. Endolysins are attractive for other fields, for example, in biotechnology for primary industry (Hoopes et al, 2009; Schmelcher et al, 2015); in food industry as the prevention against contamination by foodborne bacterial pathogens (Schmelcher and Loessner, 2016); and as a diagnostic tool to detect bacteria in different types of samples (Kretzer et al, 2007; Bai et al, 2016; Gómez-Torres et al, 2018)

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