Abstract
The paper presents the analysis of putatively complete and near-complete genomes of bacteriophages extracted from metagenomic data obtained from DNA samples isolated from Lake Baikal water using modern bioinformatic programs. A total of 73 sequences with lengths ranging from 13.8 kb to 163.7 kb belonging to phages of the Caudoviricetes class were identified. Two contigs belonging putatively to cyanophages with lengths of 36.8 kb and 163.7 kb were detected, and in the latter one an ORF with a length of 159 amino acid residues similar to the small heat shock protein (Hsp20) was identified. Analysis of the amino acid sequences identified in the assembled bacteriophage genomes using the PHROG database revealed that 27.5% of them have an unknown function, while the majority of those with similarity to known ones (23.7%) belong to the category “DNA, RNA and nucleotide metabolism”. A number of accessory metabolic genes (AMGs) were also detected in the assembled genomes: nadM, cysC, cobS, galE, cobT, etc. Most of the sequences with similarity to sequences from the IMG/VR database (89.6%) corresponded to sequences obtained from freshwater bodies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
