Abstract
Objective: The objective of this work was to screen a number of compounds for their antileishmanial efficacy and cytotoxicity profiling.Methods: Curry leaf oil, cypress oil and spikenard oil were identified by gas chromatography-mass spectrometry (GC/MS) analysis. Betulinic acid, spikenard oil, cypress oil and curry leaf oil were evaluated for their in vitro antileishmanial activity against Leishmania donovani AG83 wild-type, sodium stibogluconate resistant (SSG-resistant), paromomycin (PMM-resistant) and GE1 field type strains on axenic and cellular amastigote model and compared the results with standard drugs used to treat leishmaniasis.Results: Betulinic acid showed strong antileishmanial activity against wild-type (SI= 192.8), SSG-resistant (SI= 19.3) and GE1 strains (SI= 100), whereas cypress oil has produced highest antileishmanial activity against PMM-resistant strains (SI= 15.09) among all the tested drugs. The data obtained also revealed that cypress oil had the maximum CC50 value of 452.9 μl among all standard and tested drugs.Conclusion: All tested drugs had antileishmanial property but among them, betulinic acid possess strong antileishmanial activity in case of both wild-type and drug-resistant leishmaniasis.
Highlights
Leishmaniasis is a vector-borne parasitic disease caused by a protozoan parasite of the genus Leishmania [1]
Depending on the causative species involved, human leishmaniasis may manifest in various forms that include cutaneous leishmaniasis (CL), mucocutaneous leishmaniasis (MCL), diffused cutaneous leishmaniasis (DCL), and visceral leishmaniasis (VL), of which visceral leishmaniasis is the most lethal form of disease caused by the species of Leishmania donovani [4]
Cypress oil and spikenard oil were evaluated for the identification of the potential components by gas chromatography-mass spectrometry (GC/MS) analysis
Summary
Leishmaniasis is a vector-borne parasitic disease caused by a protozoan parasite of the genus Leishmania [1]. Depending on the causative species involved, human leishmaniasis may manifest in various forms that include cutaneous leishmaniasis (CL), mucocutaneous leishmaniasis (MCL), diffused cutaneous leishmaniasis (DCL), and visceral leishmaniasis (VL), of which visceral leishmaniasis is the most lethal form of disease caused by the species of Leishmania donovani [4]. Pentavalent antimonials such as meglumine antimoniate (Glucantime, Sanofi-Aventis) and sodium stibogluconate (Pentostan, GlaxoSmithKline) are variably effective against both VL and CL and may be administered via intravenous (IV), intramuscular (IM) or intralymphatic (IL) route. The terpenoids referred to as terpenes obtained from the plants might be an alternative source of potent new molecules for the treatment of several critical diseases since they are a rich source of therapeutically active constituents [8, 9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
