Abstract
Sequential phase-contrast photomicrography (time-lapse cinematography or periodic individual exposures) of bacterial cultures requires growth on a nutrient medium in a chamber sealed to prevent evaporation. The optical path must be continuous (no sandwich of air) from the bottom slide surface to the cover glass. We have reported a simple chamber that meets these requirements (N. N. Durham, E. C. Noller, M. W. no. 5352 1-mil FEP Teflon membrane). The membrane is eminently suitable for this use for the following reasons: (i) high oxygen permeability permits aerobic growth; (ii) low water permeability prevents drying of the agar block; (iii) high optical clarity permits photomicrography with little loss of definition; (iv) immersion oil does not wet the membrane and tracks with the objective; hence, only the area immediately
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