Sea urchin sperm head plasma membranes: characteristics and egg jelly induced Ca 2+ and Na + uptake

Abstract

Sea urchin sperm respond to egg factors with changes in the ionic permeability of their plasma membrane. It has been previously shown that plasma membranes isolated preferentially from sea urchin sperm flagella respond to egg jelly increasing their Ca 2+ and Na + uptake (Darzson et al. (1984) Eur. J. Biochem. 144, (515–522). However, the egg jelly induced acrosome reaction occurs in the sperm head, and there is evidence for an heterogeneous distribution of plasma membrane components within the various regions of this cell. We here report a method for purifying sperm head membranes using positively charged beads according to Jacobson ((1977) Biochim. Biophys. Acta 471, 331–335). Under the transmission electron microscope these membranes appeared homogeneous and apparently free of internal membranes. The yield of the preparation was 0.9% of the total protein in the sperm homogenate. The preparation contained less than 5% of the mitochondrial marker cytochrome oxidase, and 10% of the total DNA/mg protein. Surface labeling with 125I indicated a 2.5–3-fold enrichment in specific activity of the head membranes with respect to whole sperm. The SDS band pattern and the lipid composition of this preparation were different from those of isolated flagellar membranes. Phosphatidylcholine was higher in the head membranes, while phosphatidylserine and phosphatidylethanolamine were lower. The head membranes displayed a 1.7–2.3-fold higher Ca 2+-ATPase activity and a 2.5-fold lower Na +/K +-ATPase activity, than the flagellar membranes. These results are consistent with a heterogeneous distribution of membrane components along the sea urchin sperm plasma membranes. Isolated head membranes sonicated in the presence of soybean phospholipid liposomes responded to egg jelly with a species-specific increase in Ca 2+ and Na + uptake. As in whole sperm, Ca 2+ uptake was inhibited by the Ca 2+ channel blocker nisoldipine. A close analog of this compound, [ 3H]nitrendipine, binds with high affinity to head membranes in a saturable, reversible manner, showing a K d and B max of 31 nM and 5.3 pmol/mg protein, respectively.