Sea grapes powder with addition of tempe rich in collagen: An anti-aging functional food.

Rahmawati Rahmawati,Christopherous Diva Vivo,Maizer Said Nahdi,Sutamara Lasurdi Noor,Mury Kuswari,Defny Silvia Wewengkang,Hardinsyah Hardinsyah,Son Radu,William Ben Gunawan,Siti Chairiyah Batubara,Nurpudji Astuti Taslim,Happy Kurnia Permatasari,Fahrul Nurkolis,Nelly Mayulu

doi:10.12688/f1000research.55307.1

Abstract

Background: This study aimed to determine the potential anti-aging effects of sea grapes and tempe (Fermented soybeans) collagen particle size, by measuring the activities of anti-glycation, antioxidant and tyrosinase inhibitors. Methods: Collagen was isolated from sea grapes and tempe freeze dried powder and treated with different NaOH concentrations (0.10 M; 0.20 M; 0.30 M), and CH 3COOH 1 M solution, separately. The collagen particle size was adjusted by stirring at 1000 rpm for 5 and 10 hours. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to measure the antioxidant activity, and L-tyrosine and L-DOPA (l-3,4-dihydroxyphenylalanine) were used as a marker of tyrosine inhibition. Results: The collagen treated with 0.10 M NaOH produced the highest collagen yield (11.65%), and largest particle size (2455 nm). Additionally, this collagen, when treated for 5 hours, exhibited 24.70% antioxidant activity, 62.60% anti-glycation, 8.97% L-tyrosine, and 26.77% L-Dopa inhibition activities. Meanwhile, the collagen treated for 10 hours had a 9.98% antioxidant activity, 41.48% anti-glycation, 7.89% L-tyrosine, and 2.67% L-Dopa inhibition activity. Conclusion: Sea grapes and tempe collagen powder treated with 0.10 M NaOH and stirred for 5 hours, as functional foods have anti-aging properties.

Highlights

Unhealth diet and excessive exposure to UV light can cause premature skin aging, leading to excess melanin production, and darker patches (Saeedi et al, 2019)
In some Asian countries, such as Malaysia, Indonesia, and the Philippines, sea grapes or Caulerpa racemosa, which are edible marine macroalgae, are believed to be functional foods or nutraceuticals packed with antioxidant properties that can delay or prevent premature skin aging (Eren et al, 2019; Schumacker, 2015; Peñalver et al, 2020; Tanna et al, 2020; Yep et al, 2019; Pakki et al, 2020)
The collagen with the largest particle size of 2455 nm was obtained from 0.10 M NaOH treatment for 5 hours (Table 4)

Summary

Introduction

Unhealth diet and excessive exposure to UV (ultra-violate) light can cause premature skin aging, leading to excess melanin production (hyperpigmentation), and darker patches (depigmentation) (Saeedi et al, 2019). In some Asian countries, such as Malaysia, Indonesia, and the Philippines, sea grapes or Caulerpa racemosa, which are edible marine macroalgae, are believed to be functional foods or nutraceuticals packed with antioxidant properties that can delay or prevent premature skin aging (Eren et al, 2019; Schumacker, 2015; Peñalver et al, 2020; Tanna et al, 2020; Yep et al, 2019; Pakki et al, 2020). This study aimed to determine the potential anti-aging effects of sea grapes and tempe (Fermented soybeans) collagen particle size, by measuring the activities of anti-glycation, antioxidant and tyrosinase inhibitors. Results: The collagen treated with 0.10 M NaOH produced the highest collagen yield (11.65%), and largest particle size (2455 nm) This collagen, when treated for 5 hours, exhibited 24.70% antioxidant activity, 62.60% anti-glycation, 8.97% Ltyrosine, and 26.77% L-Dopa inhibition activities.

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Methods

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Conclusion