Abstract
A method is described for the synthesis and purification of the photoaffinity label Se-(8-azidoadenosyl)[ 75Se]selenomethionine. This photoaffinity label can be used to specifically and covalently label the S-adenosylmethionine binding site of proteins that use this cofactor, as exemplified by labeling of thioether methyltransferase. By utilizing the γ-emitting isotope of selenium, Se-(8-azidoadenosyl)[ 75Se]selenomethionine eliminates the need for the impregnation of acrylamide gels with fluorographic enhancers and dilution of liquid samples into scintillation cocktails, as is required with the commonly used methyl- 3H-labeled and 35S-labeled S-(8-azidoadenosyl)methionine.
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