Scutellarin Reduce the Homocysteine Level and Alleviate Liver Injury in Type 2 Diabetes Model

Yiyu Wang,Haoxin Zhang,Feng Kang,Hui Su,Kerong Jiang,Shaofang Lin,Shude Li,Biao Fan,Danshan Gu,Xiaoming Fan

doi:10.3389/fphar.2020.538407

Abstract

Scutellarin (SCU) is an active ingredient extracted from Erigeron breviscapus (Vaniot) Hand.-Mazz. Its main physiological functions are anti-inflammatory and antioxidant. In this study, we established a STZ-induced model of type 2 diabetes (T2DM) and a homocysteine (Hcy)-induced apoptosis model of LO2 to investigate whether SCU can alleviate liver damage by regulating Hcy in type 2 diabetes. Biochemical analysis indicated that SCU could improve the lipid metabolism disorder and liver function in diabetic rats by downregulating the levels of triglycerides (TG), cholesterol (CHO), low-density lipoprotein (LDL), alanine transaminase (ALT) and aspartate transaminase (AST), and by upregulating the level of high-density lipoprotein (HDL). Interestingly, SCU also could down-regulate the levels of Hcy and insulin and enhance the ability of type 2 diabetic rats to regulate blood glucose. Mechanistically, our results indicated that SCU may control the level of Hcy through regulating the levels of β-Cystathionase (CBS), γ-Cystathionase (CSE) and 5,10-methylenetetrahydrofolate (MTHFR) in liver tissue, and up-regulate folic acid, VitB6 and VitB12 levels in serum. Furthermore, SCU inhibits apoptosis in the liver of T2DM rats and in cultured LO2 cells treated with Hcy. Together, our findings suggest that SCU may alleviate the liver injury thorough downregulating the level of Hcy in T2DM rats.

Highlights

As the changes of living standard and the dietary structure, there are approximately 425 million people worldwide with diabetes (Hallberg et al, 2019b)
SCU could improve the lipid metabolism by downregulating the level of serum triglyceride (TG), cholesterol (CHO), and low-density lipoprotein (LDL) and upregulating the level of high-density lipoprotein (HDL) induced by HFHS diet and STZ treatment (p < 0.05) (Figure 1F)
The MTHFR catalyzes the conversion to tetrahydrofolate, and the methyl is bind by Hcy to form methionine

Summary

Introduction

As the changes of living standard and the dietary structure, there are approximately 425 million people worldwide with diabetes (Hallberg et al, 2019b). The diseases induced by these factors have spread all over the world, and have no trend of remission (Hallberg et al, 2019a). Approximately 79% of adults with diabetes live in low- and middle-income countries (Misra et al, 2019). Among these complications, vascular lesions are an important factor in a series of damage to tissues and organs (Wen et al, 2019). Numerous studies have shown that homocysteine (Hcy) is involved in the development and progression of many vascular complications (Hasan et al, 2019)

Methods

Results

Conclusion