Abstract
BackgroundMolecular identification based on microsatellite loci is an important technology to improve the commercial breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite subsp. formosana to further examine the transferability of across 21 Phalaenopsis species.Methods and resultsTwenty-eight polymorphic microsatellite markers were obtained using the magnetic bead enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus Phalaenopsis. The 28 newly developed polymorphic microsatellite markers with high polymorphism information content values. The best and second fit grouping (K) are inferred as two and four by the ΔK evaluation in the assignment test. This result indicates that these microsatellite markers are discernible to subgenus Phalaenopsis.ConclusionsOur results indicate that these new microsatellite markers are useful for delimiting species within genus Phalaenopsis. As expected, the genetic relationships between species of subgenus Phalaenopsis can be well distinguished based on the assignment test. These molecular markers could apply to assess the paternity of Phalaenopsis as well as investigating hybridization among species of genus Phalaenopsis.
Highlights
Molecular identification based on microsatellite loci is an important technology to improve the com‐ mercial breeding of the moth orchid
Our results indicate that these new microsatellite markers are useful for delimiting species within genus Phalaenopsis
The genetic relationships between species of subgenus Phalaenopsis can be well distinguished based on the assignment test
Summary
Molecular identification based on microsatellite loci is an important technology to improve the com‐ mercial breeding of the moth orchid. Formosana, commonly known as the Taiwan moth orchid, has been widely used as an important breeding hybrids parent, and it is one of the most important progenitors for the traits of modern large and white of floral organs commercial hybrids breeding (Tanaka et al 2005). Phalaenopsis equestris is another important breeding parent for the miniature type of multi-flowers and artificial hybrids with white petals and sepals and a red lip (Men et al 2003; Tang and Chen 2007)
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