Screening the Antibacterial Activity of Garlic (Allium sativum) Extract against Methicillin- Resistant Staphylococcus aureus (MRSA)

Abstract

The growth of human skin pathogens or kill them with no toxicity to host cells are considered candidates for developing new antimicrobial drugs. Consequently, there is a critical need to research for new antimicrobial agents with promising natural activities to provide an alternative to common antibiotics. The isolation of Staphylococcus aureus pus samples from wounds was used. Totally 52 pus samples were collected from Government Hospital in Virudhunagar. Fluid thioglycolate medium was used as an enriched medium for S. aureus. The methanol extract of Allium satiuvm were screened for antibacterial activity against isolated Staphylococcus aureus. The maximum zone of inhibition in methanol extract is 11 mm/30 ul (SA5) and in methanol extract is 7 mm/30 ul (SA10). These strains SA5 and SA10 denotes isolated S.aureus strains numbers. Some of the strains are not responded to the plant extracts so, as the concentration of the extract increases the zone of inhibition can also be increased. The minimum inhibitory concentrations of the methanol extract of Allium satiuvm and were determined. But, 40 mg/ml concentration inhibited the growth.