Abstract

The aliphatic secondary and tertiary amines react with tropaeolin 00 to form chloroform soluble compounds. This reaction was applicable to a screening test for methamphetamine in the urines. The experimental procedure is as follows : 5 ml of the urine sample is added to a tropaeolin 00 solution. The mixture is allowed to stand for 2 minutes and then added 5 ml of chloroform. After shaking for 30 seconds, the chloroform layer is separated and dehydrated by anhydrous sodium sulfate, and methanol solution of sulfuric acid is added to the chloroform layer. The red or reddish-violet color of the chloroform layer suggests the presence of methamphetamine. The limit of identification for methamphetamine was 3 μg/ml of urine. No detectable interference was observed to identify methamphetamine in the presence of such drugs as barbiturates, analgesics, antacids, and vitamins.

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