Abstract
This paper focuses on determination of the influence of electrochemically active microorganisms on the transmission of electrons from the respiratory enzymes to the electrode and assembling of exoelectrogens to the simulated wastewater medium. In this study, the total of eight microorganisms were experimentally tested to exhibit growth and high iron-reducing ability in the absence of mediators. A major connection was observed between the growth and iron-reduction ability of the microorganism. The growth and iron-reduction ability were monitored experimentally over time. Based on output data, the screening was done among eight different microorganisms, where Escherichia coli -K-12 was chosen as the most potent microorganism for its wide application in a microbial fuel cell (MFC). In the present study, various biochemical process factors were optimized statistically using Taguchi methodology for the rapid development of growth and iron-reducing assay conditions. The design of various experimental trials was carried out using five process factors at three levels with orthogonal arrays (OA) layout of L18. Five process factors, including quantity of lactose, volume of trace element solution, inoculum percentage, pH, and temperature, were taken into consideration as imperative process factors and optimized for evaluation of growth of bacteria and iron reduction ability. The larger-is-best signal to noise (S/N) ratio, together with analysis of variance ANOVA, were used during optimization. Anticipated results demonstrated that the enhanced bacterial growth of 124.50 % and iron reduction ability of 112.6 % can be achieved with 8 g/L of lactose, 2 ml of trace element solution, 4 % (v/v) of inoculum, pH 7, and temperature of 35 oC. Furthermore, the growth and iron reduction time profiles of Escherichia coli-K12 were performed to determine its feasibility in MFC. Open circuit voltage of 0.555 V was obtained over batch study on a single chamber microbial fuel cell (SCMFC).
Highlights
It is a conventional statement that renewable energy sources are urgently necessary
The exception was found in the case of Escherichia coli -K-12 [40]
The current study investigates optimization of process factors, to get the progress of quick growth and iron reduction assay for Escherichia coli -K-12 using Taguchi methodology
Summary
It is a conventional statement that renewable energy sources are urgently necessary. The present necessity for fossil fuels is unsustainable due to their toxic waste and restricted supply [1]. One of the main strategies of using renewable energy sources and applying power-saving programs does not decrease focus on cutting the energy demand but increases the demand for stand-alone systems as alternates. Use of renewable energy in the world increases in order to have a more supportable energy mix, which reduces greenhouse gas emissions, and allows lower dependency on fossil fuels [2]. Much research is conducted in favor of renewable energy sources as alternative solutions, no one can completely replace fossil fuels. This proves that different alternative renewable sources are greatly needed to meet the energy demand
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Electrochemical Science and Engineering
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
