Screening sugarcane somaclones and their parent varieties against red rot (Colletotrichum falcatum) and assessment of Variability by RAPD and SSR markers

Km Nasiruddin,Kuasha Mahmud,L Hassan,Ma Hossain

doi:10.3329/sja.v13i2.26578

Abstract

The experiment was conducted to find out red rot disease causing pathogenic reactions of somaclones (R0) and their respective parents at BSRI farm in the cropping season 2011-2012. A total number of sugarcane genotypes 24 including 4 sugarcane varieties viz. Isd 37, Isd 38, Isd 39 and also Isd 40 were inoculated to screen red rot resistant levels (R = Resistant, MR = Moderately Resistant) to susceptible levels (S = Susceptible, MS = Moderately Susceptible, HS = Highly Susceptible) after 7 months of planting. As a result, Isd 37 variety (source/parent) and its somaclones CC3712 and CC3786 were found to be resistant while 7 somaclones showed moderately resistant reaction. Furthermore, somaclones of Isd 38 variety viz. CC382 as moderately resistant, CC3810 as moderately susceptible and also CC11(38)8 as susceptible reaction were recorded while Isd 38 variety showed resistant reaction. On the other hand, Isd 39 and Isd 40 source varieties with their somaclones were found as resistant reaction against red rot pathogen. Some somaclones showed different reaction from their source varieties such as moderately resistant somaclones were obtained from resistant source variety Isd 37 while somaclones CC382 as moderately resistant, CC3810 as moderately susceptible and somaclone CC388 as susceptible were obtained from resistant source variety Isd 38 against red rot pathogens respectively. Besides, some somaclones showed similar reaction from their resistant source varieties Isd 37, Isd 39 and also Isd 40. It revealed that reaction against red rot pathogen, induced somaclones showed variation with their source varieties. Red rot resistance somaclones were isolated and assessed for the presence of variability through RAPD and SSR markers. Cluster and sub cluster formation verified the presence of variability in the red rot resistance somaclones with respect to the parent.SAARC J. Agri., 13(2): 173-182 (2015)

Highlights

Worldwide, Sugarcane (Saccharum officinarum L) is a major source of commercial sugar along with many other value added products and by-product
Reaction of inoculated fungus for red rot disease on four varieties and their somaclones are shown in table 3
Four varieties namely Isd 37, Isd 38, Isd 39, Isd 40 and their 20 somaclones were inoculated for red rot disease

Summary

INTRODUCTION

Sugarcane (Saccharum officinarum L) is a major source of commercial sugar along with many other value added products and by-product. Considerable progress has been made in the mapping of the sugarcane genome and its progenitors (Aitken et al, 2005) Somaclones and their sources varieties were screened for their reaction to isolates of red rot pathogen to get resistant genotypes. Screening of sugarcane genotypes against red rot pathogen is a pre-requisite in the varietal improvement. This experiment was conducted to find out the level of resistance of induced somaclones and their sources varieties against red rot pathogen (Colletotrichum falcatum Went.). Ten healthy canes of each somaclone and their sources varieties were inoculated by spore suspension (spore conc.106 ml-1) of red rot disease causing fungus (colletotrichum falcatum) with the help of hypodermic syringe method. Dendrograms were constructed by using Uweighted Pair Group Method of Arithmetic Means (UPGMA) algorithm (Sneath and Sokal, 1973) provided in the software (Statistica computer package 2006, STA_CLU)

RESULTS AND DISCUSSIONS

CONCLUSION

22. Standard Variety Isd 40