Abstract
Soybean is an important source of plant protein used in human diets. Cadmium (Cd) from agricultural soil is a toxic metal for plants, including soybean. Furthermore, the cadmium is taken up to seeds of soybean and becomes a risk for animals and human by feed and food chain. A method to avoid this risk is represented by the selection and breeding soybean lines/cultivars with genetic potential to minimized cadmium accumulation. This work reports an approach assisted by DNA-markers for selection of soybean cultivars with low Cd-accumulation. In this study, we screened the alleles of Cda1 gene, in 22 soybean cultivars, using three DNA markers (SSR-Sack149, Gm-dCAPS-HMA1 and Cda1-KASP). The results showed that 11 genotypes carried the allele for low cadmium accumulation, 8 genotypes had the allele for high cadmium accumulation and three genotypes were heterozygous/heterogenous. All three markers classified the soybean genotypes in the same manner but KASP marker is more efficient in marker-assisted selection/breeding for Cda1. This study offers valuable information to breeders and other researches regarding the selection of soybean germplasm with low cadmium accumulation in the pursuit of reducing the Cd-accumulation and assuring the food safety worldwide.
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