Abstract
The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.
Highlights
Colorectal cancer is one of the most common malignant tumors of the alimentary tract, which is strongly invasive in the progressive stage, hard to achieve radical cure, and has a high post-operative recurrence rate (Xu et al, 2006)
Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library
LoVo cells were used as the target cells and normal human colorectal mucous epithelial (NHCME) cells as the absorber cells for subtraction biopanning from a c7c phage display peptide library for screening polypeptides binding to colorectal cancer cells and using them as vectors for colorectal cancer targeting therapy
Summary
Colorectal cancer is one of the most common malignant tumors of the alimentary tract, which is strongly invasive in the progressive stage, hard to achieve radical cure, and has a high post-operative recurrence rate (Xu et al, 2006). The traditional chemotherapeutic agent for the disease has weak tissue selectivity for colorectal tissue, which makes it hard to achieve an effective killing concentration at the site of the tumor. Phage display peptide library technology is gradually becoming a hot topic for research in tumor targeting therapy (Landon et al, 2004; Samoylova et al, 2006). LoVo cells were used as the target cells and normal human colorectal mucous epithelial (NHCME) cells as the absorber cells for subtraction biopanning from a c7c phage display peptide library for screening polypeptides binding to colorectal cancer cells and using them as vectors for colorectal cancer targeting therapy. The study provides a new experimental basis for clinical research in the mechanism underlying the genesis and development of colorectal cancer, and for the development of new targeting agents
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