Screening of Volatile Constituents of N. sativa on Calcium Depleted Heart Model

Abstract

The number of deaths from cardiovascular disorders is rising every year. Nigella sativa, one of the accessible natural plants, has a broad range of pharmacological effects. The Nigella sativa seeds were removed, cleaned, and preserved in order to research the effects of the plant on the cardiovascular system. After being ground into a fine powder, the seeds were used to extract the volatile oil from the seeds using the steam distillation process. The hypodynamic model was used after the rat's heart was removed. Using the standard kreb's hanselet salt solution to mount the isolated heart, a typical graph was produced. As the calcium concentration was reduced, the graph's negative tropic activity became apparent. When N. sativa was administered it has produced additional negative tropic effect on hypodynamic heart. At 0.1 ml produced negative tropic effect and keeps on decreasing as the dose increases by 0.2, 0.4, 0.8ml in dose dependent manner.