Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

Ramil Vafin,Nuraniya Vasilova,Damir Askhadullin,Danil Askhadullin,Irina Rzhanova

doi:10.5586/aa.1746

Ramil Vafin, Nuraniya Vasilova + Show 3 more

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https://doi.org/10.5586/aa.1746

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Abstract

Screening genotypes of wheat by allelic variants of Waxy and HMW-GS genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of Triticum aestivum L. with valuable alleles of Waxy and HMW-GS genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of Waxy and HMW-GS genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (Wx-A1b and Wx-B1b) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of HMW-GS, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify Waxy and HMW-GS alleles are effective methods of evaluating their allelic polymorphism.

Highlights

Screening wheat genotypes for allelic variants of Waxy [1,2,3] and HMW glutenin subunits (HMW-GS) [4,5,6] is an integral part for marker-assisted selection of varieties with high value as indicators for flour-baking and technological properties of the grain
As a result of the DNA testing of the 70 samples in order to identify T. aestivum genotypes by allelic variants of Waxy genes, it was established that 46 samples (65.7%) had a combination of active alleles Wx-A1a/B1a/D1a, two samples: lines K-139-08-1 and K-311-08-1 (2.9%) – combination Wx-A1g/B1a/D1a, by the classification of wheat types with various content of Waxy genes, 10 samples (14.3%) belonged to the second type (Wx-A1b/B1a/D1a), eight samples (11.4%) – to the third type (Wx-A1a/B1b/D1a), three samples: lines K-243-13Wx-2, K-243-13Wx-4, and K243-13Wx-6 (4.3%) – to the seventh type (Wx-A1b/B1b/D1a)
The new genotypes of T. aestivum presented in this study expand the circle of donors and sources of economically-valuable alleles of Waxy and HMW-GS genes which indicates the importance of the results of our research reported here

Summary

Introduction

Screening wheat genotypes for allelic variants of Waxy [1,2,3] and HMW glutenin subunits (HMW-GS) [4,5,6] is an integral part for marker-assisted selection of varieties with high value as indicators for flour-baking and technological properties of the grain. The systems of molecular marking of allelic variants of Waxy [1,12,13] and HMW-GS [6,10,14] of wheat using DNA technologies selected for allelic polymorphism of genes analyzed make it possible to identify Triticum aestivum L. genotypes and so expand the range of donors and sources of economically-valuable alleles. The purpose here is to study, preserve, and apply to the biodiversity of cultivated plant forms, as well as managing selection of new genotypes with high economical productivity and resistance against bio- and abiostressors

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